Sina Azad; hamid Amanlou; Najme Eslamian Farsuni; Tahere Amirabadi Farahani; Mohammad hadi Khabbazan
Volume 25, Issue 1 , April 2023, , Pages 37-50
Abstract
In the current study, the effect of source and level of copper in the diet on production and health of dairy cows using 105 multiparous pregnant Holstein cows from -21 until +15 days relative to calving in randomized complete block design with 3 treatments and 35 replications were investigated. The experimental ...
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In the current study, the effect of source and level of copper in the diet on production and health of dairy cows using 105 multiparous pregnant Holstein cows from -21 until +15 days relative to calving in randomized complete block design with 3 treatments and 35 replications were investigated. The experimental treatments include: 1) diet containing copper at the NRC recommended levels from copper sulfate source (NRC-S), 2) diet containing copper at twice the NRC recommended levels from copper glycinate source (2NRC-Gly) and 3) diet containing copper at twice the NRC recommended levels from copper sulfate source (2NRC-S). Milk yield and composition were not affected by experimental treatments, but treatment by time interaction showed that cows fed by 2NRC-Gly had more milk than NRC-S group (P<0.05) at 60, 90,120 DIM and cow in 2NRC-Gly produced more milk at 90 and 120 days in milk compared to NRC-S (P<0.05). The somatic cells count for 2NRC-Gly cows was lower compared to NRC-S cows (P 0.05). The incidence of subclinical mastitis at 15 DIM in 2NRC-Gly was lower compared to the other two treatments (P = 0.05). No difference in body weight and body condition score changes were observed across treatments. Blood metabolites and liver enzymes were not affected by adding different Cu sources, but serum albumin postpartum was increased in 2NRC-Gly group relative to the other two groups (P 0.05). Based on the results, adding copper especially by copper glycinate source at twice the NRC recommended levels led to an increase in the serum albumin concentration, a decrease in milk somatic cells count and lower incidence of subclinical mastitis, which could indicate an improvement in health of cows during transition period.
Fateme Ahmadi; hamid Amanlou; Tahere Amirabadi Farahani; Najme Eslamian Farsuni
Volume 24, Issue 4 , December 2022, , Pages 415-425
Abstract
The aim of the present study was to evaluate the effect of supplementing close-up diets of Holstein dairy cows with rumen undegradable protein (RUP) on milk yield, calf birth weight and colostrum composition during the hot season. Eighty-eight multiparous Holstein dairy cows were enrolled in the study ...
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The aim of the present study was to evaluate the effect of supplementing close-up diets of Holstein dairy cows with rumen undegradable protein (RUP) on milk yield, calf birth weight and colostrum composition during the hot season. Eighty-eight multiparous Holstein dairy cows were enrolled in the study 30 d before expected calving and assigned to 1 of 2 treatments groups: low crude protein (14.3% CP with 4.0% RUP based on DM) and high crude protein (17.1% CP with 6.0% RUP based on DM). Diets were fed for ad libitum intake during the close-up and fresh period. After calving, all cows received the same diet from calving to 21 days of milk. Milk production of all cows was recorded daily from the day of calving to 21 days after calving. The milk samples of each cow were collected twice a week to measure milk composition. The evaluation of body condition score (BCS) was performed at -30, -3, 0, and 21 d relative to calving. The production of milk and the amount of milk protein and lactose were greater and milk fat content was lower in cows fed high protein diet than cows fed low protein diet (P<0.05). The BCS changes were not different between cows of two treatments in pre- and post-partum periods. According to the results, feeding a diet containing high RUP content to close-up cows during the hot season increases milk yield and decreases milk fat percentage in fresh cows.
Fateme Ahamadi; hamid Amanlou; Najme Eslamian Farsuni; Tahere Amirabadi Farahani
Volume 23, Issue 3 , September 2021, , Pages 363-374
Abstract
The objective of this study was to investigate the effect of decreasing dietary protein content with supplementing protected methionine (pMet) and lysine (pLys) on intake, milk yield, metabolic status, and apparent digestibility of nutrients in Holstein fresh cows. Immediately after calving, 16 cows ...
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The objective of this study was to investigate the effect of decreasing dietary protein content with supplementing protected methionine (pMet) and lysine (pLys) on intake, milk yield, metabolic status, and apparent digestibility of nutrients in Holstein fresh cows. Immediately after calving, 16 cows with the similar BCS and parity were randomly assigned to two treatments. Experimental diets provided equal amounts of lysine (132 to 133 g/d) and methionine (40 to 43 g/d). The treatments were: a diet containing 18.35 % CP without supplementing pMet and pLys (HP); and a diet containing 16.45 % CP with supplementing rumen-protected amino acids (LPML). Individual dry matter intake and milk yield were recorded daily during fresh period. Blood samples were collected at 0, 3, 7, 14 and 21 d after calving to determine the serum concentrations of mineral and metabolites. Feed intake was not affected by experimental diets but milk yield was higher for HP cows than cows in LPML (P < 0.01). The yield of milk protein, lactose, fat and concentration of milk urea nitrogen (MUN) were higher in HP cows compared to LPML (P < 0.05). Compared to HP cows, cows in LPML had lower blood urea nitrogen (BUN) and higher serum concentrations of total protein and globulin (P < 0.01). Based on the results of the present study, reducing dietary protein content while maintaining the supply of limiting amino acids decreased lactational performance and feed efficiency of Holstein fresh cows.
salman afshar; hamid Amanlou; Davood Zahmatkesh
Volume 23, Issue 2 , July 2021, , Pages 201-212
Abstract
The effect of using whole safflower seed and flaxseed in the during transition period on performance, digestibility, production and composition of colostrum and blood parameters of Afshari ewes using three treatments and 27 pregnant ewes with average age of three years, weight of 85 ± 1.28 ...
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The effect of using whole safflower seed and flaxseed in the during transition period on performance, digestibility, production and composition of colostrum and blood parameters of Afshari ewes using three treatments and 27 pregnant ewes with average age of three years, weight of 85 ± 1.28 kg and range 6 weeks before expected lambing in a completely randomized design was investigated. Experimental treatments included; 1- control diet (base), 2- diet containing 8% safflower seed, 3- diet containing 8% flaxseed. The effect of treatments was not significant on functional traits of ewes pre-lambing but their dry matter intake and weight change post lambing were affected by safflower seed and flaxseed treatment (P< 0.05). The experimental treatments were not significant on pre-lambing digestibility coefficients, composition and amount of colostrum and milk as well as lamb performance. The triglyceride, cholesterol and high-density lipoprotein levels in blood plasma of flaxseed-fed ewes were higher than safflower and control treatments in pre-lambing. The results demonstrated that using safflower seed and flaxseed in the period close to lambing did not have negative effects on the performance of gestation ewes but increased their dry matter and improved the health of lactating ewes and lambs by preventing severe weight loss in post lambing.
Mehrdad Shahizad; hamid Amanlou; Najme Eslamian Farsuni; Tahere Amirabadi Farahani; Hadi Khabazan
Volume 22, Issue 3 , September 2020, , Pages 349-356
Abstract
The objective of present study was to investigate the effect of different levels of sesame meal in early lactation cow's ration on dry matter intake, milkyield and composition, apparent digestibility of nutrients and blood metabolites. Six dairy lactating Holstein cows with average DIM 26± 3 (n=3primiparous ...
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The objective of present study was to investigate the effect of different levels of sesame meal in early lactation cow's ration on dry matter intake, milkyield and composition, apparent digestibility of nutrients and blood metabolites. Six dairy lactating Holstein cows with average DIM 26± 3 (n=3primiparous and n=3 multiparous cows) were randomly assigned to 1 of 3 experimental treatments (different levels of sesame meal) in repeated 3×3Latin square design. Experimental treatments were control (without sesame meal), 4.33% sesame meal and 8.66% sesame meal (DM basis). Theexperimental periods consisted of 21 d with the first 14 days as adaptation and the last 7 days as sampling days. Increasing level of sesame meal inearly lactation rations did not affect dry matter intake, milk yield and composition (fat, protein and lactose), feed utilization efficiency, apparentdigestibility of nutrients and blood metabolites (P 0.05), while milk urea nitrogen decreased with increasing the level of sesame meal (p < 0.05).Blood urea nitrogen concentrations also tended (P = 0.1) to decrease with increasing levels of sesame oil. In general, the lack of response in feedintake and milk yield with increasing the levels of sesame meal in early lactation cow's ration showed that sesame meal could be a proper proteinsource in early lactation cow's ration.
gholamhosein Irajian; hamid Amanlou; Asadollah Teimouri Yansari; hamid reza mirzaie almouti; hormoz mansouri
Volume 19, Issue 4 , March 2018, , Pages 803-818
Abstract
This study investigated the effects of alfalfa hay and corn silage particle size on chewing activity, passage rate of solid fraction in the rumen and performance of pregnant ewes. The experiment has been done using 30 ewes (35±0.4 months) in a completely randomized design with five treatments ...
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This study investigated the effects of alfalfa hay and corn silage particle size on chewing activity, passage rate of solid fraction in the rumen and performance of pregnant ewes. The experiment has been done using 30 ewes (35±0.4 months) in a completely randomized design with five treatments and six replicates during a 30 days period. Forages were including alfalfa hay (three sizes, geometric mean 2.42, 4.91 and 7.04 mm) and corn silage (two sizes, geometric mean 3.36 and 7.73 mm). Treatments including fine particles of alfalfa hay and corn silage had the least physical effectiveness factor (pef > 8) and pef values (pef >8, pef >1.18) were reduced with decrease in particle size (p<0.05). Geometric mean also decreased (p<0.05). Following reduction in particle size of forages, passage rate of solid fraction in the rumen and terminal part of digestive tract (TDT) were increased (p<0.05) and retention time in the rumen, TDT and total retention time were decreased (p<0.05). Feed intake time, chewing activity and total chewing time were not affected (p<0.05). Results showed that it is necessary to consider the appropriate balance between particle size as a representative of the physical and chemical characteristics of the diet. The treatment of alfalfa fine particle size plus corn silage long particle size in most cases showed better results, and it seems that this treatment has provided a more appropriate balance in the diet for feeding ewes. Therefore, in preparing the pregnant ewes’ diet, we can expect better performance by considering the particle size.
hamid amanlou; abolfazl zali
Volume 19, Issue 2 , August 2017, , Pages 337-348
Abstract
The aim of this study was to evaluate the effects of Pomegranate seed pulp (PSP) containing long-chain fatty acids with L-carnitine (LC) on performance and carcass traits of thirty two Mahabadi male kids. Experimental diets were 1) diet without PSP and LC 2) diet with 10% PSP Kg/DMI and without ...
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The aim of this study was to evaluate the effects of Pomegranate seed pulp (PSP) containing long-chain fatty acids with L-carnitine (LC) on performance and carcass traits of thirty two Mahabadi male kids. Experimental diets were 1) diet without PSP and LC 2) diet with 10% PSP Kg/DMI and without LC 3) diet without PSP and with 300ppm LC Kg/DMI and 4) diet with 10% PSP and 300ppm LC Kg/DMI. Kids were slaughtered at the end of study and carcass characteristics were measured. Addition of PSP and LC in diet decreased kidney fat (P<0.05). The kids fed with PSP and LC supplement had higher fat digestibility than other treatments (P<0.05). Furthermore, the use of PSP in the diet decreased the feed cost per Kg of hot carcass (P=0.05). The results of this study indicated that replacing a part of dietary cereal grains with PSP and LC supplement decreased kidney fat. Also, the simultaneous use of LC and PSP increased apparent digestibility of fat, and showed numerically higher performance. According to the results of this study, the PSP can be used as an economical byproduct without negative effects on performance, and along with LC in the diet of kids it will reduce kidney fat as well.
Seyyed Saeid Mousavi; Hamid Amanlou; Ali Nikkhah; Hamid Reza Mirzaei Alamouti; Ali Moustafa Tehrani
Volume 18, Issue 3 , October 2016, , Pages 413-430
Abstract
The aim of this study was to investigate the effects of different metabolizable protein levels in diet during the peri parturition on performance and blood parameters of Afshari ewes and lambs. Thirty two once lambing ewes in the last six weeks of pregnancy and the first six weeks of lactation with diets ...
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The aim of this study was to investigate the effects of different metabolizable protein levels in diet during the peri parturition on performance and blood parameters of Afshari ewes and lambs. Thirty two once lambing ewes in the last six weeks of pregnancy and the first six weeks of lactation with diets before and after parturition containing rates of NRC recommended and 20% higher one as low and high amount of metabolizable protein respectively requirements of ewe with two lambs as Factorial experiment in a completely randomized design were fed. Dry matter intake, body weight, body condition score ewes and their changes, weight lamb in the first and third weeks, and daily weight gain of lambs were not affected by treatments. Amount and composition of the colostrum were not affected by the treatments, but milk yield increasing was observed at low metabolizable protein before and after lambing treatment(p=0.007) as compared to other treatments. Urea (P = 0.0001) and cholesterol (P = 0.02) of plasma were significantly higher in high metabolizable protein than the low metabolizable protein in late gestation. Reducing insulin resistance (P = 0.03) and increasing insulin sensitivity (P = 0. 01) were observed in the treatment low metabolizable protein before and after lambing. No significant difference was observed on the numbers of white blood cells, red blood cells and other blood cells between the treatments. The results of this study, the percentage of metabolizable protein by the NRC for Afshari ewes during late gestation and early lactation is advisable.
Hamid Amanlou; Hadi Shafiei; Tahereh Amirabadi Farahani; Farshid Kheiri; Najme Eslamian Farsuni
Volume 18, Issue 1 , April 2016, , Pages 27-38
Abstract
In order to compare the precision of predictions of NRC (2001) and CNCPS models, 17 Holstein fresh cows were assigned in a randomized complete block design to a balanced diet with NRC (2001) and CNCPS software. The predicted DMI, energy allowable milk and protein allowable milk by both models were compared ...
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In order to compare the precision of predictions of NRC (2001) and CNCPS models, 17 Holstein fresh cows were assigned in a randomized complete block design to a balanced diet with NRC (2001) and CNCPS software. The predicted DMI, energy allowable milk and protein allowable milk by both models were compared with observed values. The precision of the predictions of models was evaluated by calculation of R2, mean bias and root mean square prediction error (RMSPE). Both models predicted DMI lower than the observed values (P<0.01). The mean bias, RMSPE and R2 were 2.22, 2.84 and 0.48 for NRC (2001) and 3.18, 3.99 and 0.52 for CNCPS, respectively and CNCPS predicted the DMI 20 percent lower than the observed value. The observed milk production was 29.78 kg/d. predicted energy allowable milk by NRC (2001) and CNCPS were 25.68 and 22.32 kg/d, respectively. Predicted protein allowable milk with NRC (2001) and CNCPS were 25.80 and 28.37 kg/d, respectively and NRC (2001) predicted the milk production 14 percent lower than observed value. Given the importance of DMI in fresh cows seems NRC (2001) has more precision compared to CNCPS for estimating nutritional requirements.
Moustafa Hajilou; Hamid Reza Mirzaei Alamouti; Mehdi Ganjkhanlou; Hamid Amanlou
Volume 17, Issue 2 , October 2015, , Pages 347-358
Abstract
Four ruminally-cannulated Holstein cows were used in a 4 × 4 Latin square changeover design within periods of 21 days to evaluate effects of feeding frequency of different sources of rumen degradable nitrogen on total tract digestibility of nutrients, feeding behaviors, rumen and blood metabolites ...
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Four ruminally-cannulated Holstein cows were used in a 4 × 4 Latin square changeover design within periods of 21 days to evaluate effects of feeding frequency of different sources of rumen degradable nitrogen on total tract digestibility of nutrients, feeding behaviors, rumen and blood metabolites of Holstein cows. Treatments were: 1) control diet which was as total mixed ration (TMR) offered once daily in the morning (08:00), 2) control diet in which a part of its soybean was offered at 19:00, 3) control diet in which a part of its urea was offered at 19:00 and 4) control diet in which a part of its fish meal was offered at 19:00. Rumination time increased in cows fed twice daily with protein sources (P<0.05). Total tract digestibility of dry matter and organic matter were higher in cows received urea at the evening time. Total tract digestibility of neutral detergent fiber increased with feeding all sources of protein at the evening time (P<0.05). Molar proportion of volatile fatty acids, rumen pH and rumen ammonia concentration were not affected by feeding time of protein sources. The results indicated that increasing delivery of rumen degradable nitrogen sources improved dry matter and fiber digestibility and chewing activity of non-lactating Holstein dairy cows.
Mohammad Mehdi Moeini; Mojgan Azari Torbat; hamid Amanlou
Volume 12, Issue 2 , October 2010, , Pages 51-59
Abstract
15 Holstein cows (1st lactation, 554 ± 57 Kg) randomly divided into three groups. Cows fed Vicia ervilia seed at the rate of 0%, 7% and 14%, DM of dietary, which replaced with Soya been meal and barely. Vicia ervilia (VE) contain approximately: 93.93% DM, 22.8% CP, 5.95% Ash, 3.02% EE, 63% NFE, ...
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15 Holstein cows (1st lactation, 554 ± 57 Kg) randomly divided into three groups. Cows fed Vicia ervilia seed at the rate of 0%, 7% and 14%, DM of dietary, which replaced with Soya been meal and barely. Vicia ervilia (VE) contain approximately: 93.93% DM, 22.8% CP, 5.95% Ash, 3.02% EE, 63% NFE, 5.23% CF, 15.01% NDF and 6.13% ADF. The effective ruminal degradability of CP and DM of VE amounted to 74.76% and 70.57% respectively. Milk production increased in 14% group compared with control (P< 0.05). The values of urea nitrogen in treated cows were significantly increased (P< 0.05). The result of this study indicated that the Vicia ervilia can be used as a protein source replaced with Soya been at the 14% level in dairy cows ration during lactation period after partition.